Which of the following best defines a prokaryotic cell?

A prokaryotic cell is best defined as a cell without a membrane-bound nucleus. This fundamental characteristic distinguishes prokaryotic cells from eukaryotic cells, which do have a defined nucleus enclosed by a nuclear membrane. In prokaryotes, such as bacteria and archaea, the genetic material is located in a region called the nucleoid, which is not separated from the rest of the cell by membranes.

Furthermore, prokaryotic cells are generally simpler in structure compared to eukaryotic cells, lacking many of the organelles typically found in eukaryotes, such as mitochondria and endoplasmic reticulum. They do contain DNA, so the option referring to a cell that does not contain DNA is also not applicable. Understanding these distinctions is crucial in cell biology, as it forms the basis for various biological processes and the classification of life.